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KMID : 1004820090100020059
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Journal of Biomedical Research
2009 Volume.10 No. 2 p.59 ~ p.67
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2,4-bis(p-hydroxyphenyl)-2-butenal induces apoptotic cell death and G₂/M phase arrest via inactivation of NF-κB in human colon cancer cells
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Ban Jung-Ok
Lim Tae-Hong
Kim Dae-Hwan
Kim Yun-Bae
Nam Sang-Yoon
Hong Jin-Tae
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Abstract
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The Maillard Reaction Products (MRPs) such as Glucose-tyrosine (Glu-Tyr) and Xylose-arginine(Xyl-Arg) have antioxidant, antimutagenic, and antibacterial effects. However, to date, still little is known aboutthe other biological effects of the MRPs. In this study, we investigated whether the fructose-tyrosine MRP,2,4-bis(p-hydroxyphenyl)-2-butenal (Fru-Tyr), could modulate cell cycle progression and NF-κB activity, andthereby induce apoptotic cell death of colon cancer cells. Treatment with different concentrations (10-40 μg/ml) of Fru-Tyr for 24 h inhibited colon cancer cell (SW620 and HCT116) growth followed by induction ofG2/M phase cell cycle arrest and apoptosis in a dose-dependent manner. We also found that Fru-Tyr suppressedtumor necrosis factor-alpha (TNF-α)-induced NF-κB transcriptional activity. Moreover, Fru-Tyr induced theexpression of apoptotic gene, cleaved caspse-3. These results suggest that Fru-Tyr inhibited colon cancer cellgrowth through induction of G2/M phase cell cycle arrest and apoptotic cell death by modulating of NF-κB.
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KEYWORD
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2,4-bis(p-hydroxyphenyl)-2-butenal, Fru-Tyr, Cell cycle, NF-κB, Colon cancer cells
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